NGS Analysis

Aptamer Next Generation Sequencing

We prepare multiple libraries for simultaneous sequencing analysis on an Illumina HiSeq 2500. We capture at least 1.000.000 sequences per library.  We assess library complexity based on distribution of copy number of these aptamer sequences. NeoVentures has developed proprietary software to characterize enrichment rates and identify candidate aptamers. We differentiate sequences that continue to curve upwards vs. those that plateau as seen in the figure below. Sequences that plateau generally have lower binding affinities.

 

aptamer next generation sequencing